Target self-initiation nucleic acid coding amplification (TNEA) is a method that uses target molecules to trigger nucleic acid coding amplification reaction, so as to realize the amplification detection of target molecules. TNEA can be combined with fluorescent probes, nanomaterials, biosensors, etc., to form a high sensitivity and high specificity detection platform for detecting various bioanalytical targets, such as bacteria, viruses, proteins, nucleic acids, etc.

Accurate imaging analysis of multiplex mRNA based on target self-initiated nucleic acid coding amplification refers to the use of TNEA technology to realize the simultaneous detection and localization of multiple mRNA in cells. The mRNA is a nucleic acid molecule carrying genetic information involved in protein synthesis and regulation. The expression level and distribution pattern of mRNA are closely related to the function and state of cells, so the accurate imaging analysis of mRNA is important for revealing the biological mechanism of cells and the diagnosis of diseases.

The multiplex mRNA accurate imaging analysis method of TNEA technology enables the simultaneous detection and localization of four types of mRNA (c-Myc, Klf 4, Oct4 and Sox 2). This method uses four different colored fluorescent probes (FAM, Cy 3, Cy 5 and Cy 7) to bind to the complementary sequences of the four mRNA, and generates a strong fluorescence signal through the TNEA reaction. By fluorescence microscopy, the expression level and distribution patterns of the four mRNA were clearly distinguished and were found to be significantly different in normal and cancer cells.

Multiplex nucleic acid imaging methods based on DNA hybrid chain reaction (HCR) and quantum dots (QDs) enable the simultaneous detection and localization of three cellular mRNA species (β -actin, GAPDH and survivin). This method uses three different color QDs (red, green, and blue) to bind to the complementary sequence of the three mRNA, and generates long-chain DNA through HCR reaction, thus magnifying the fluorescence signal. By fluorescence microscopy, the three mRNA expression levels and distribution patterns can be clearly distinguished and found to be significantly different in normal and cancer cells.

Reference Documentation:

[1]Target-Triggered Nucleic Acid Encoding Amplification for Multiplex mRNA Precise Imaging Analysis.

[2]Multiplexed imaging of nucleic acids in living cells using DNA hybridization chain reaction and quantum dots.