The blood genome extraction kit provides a fast and flexible method for fresh or frozen whole blood (samples treated with anticoagulants such as citrate, EDTA or heparin) to extract total DNA, including genomic DNA and mitochondrial DNA . This product can flexibly process 0.1-20ml whole blood, using a non-spin column method, without the use of phenol, chloroform and other organic solvents, effectively removing protein, pigment, lipid and other inhibitory impurities pollution. The entire process is operated in one tube, reducing the risk of contamination and sample confusion. The extracted DNA has high yield and good quality, and can be directly used in experiments such as PCR, fluorescent quantitative PCR, enzyme digestion, Southern Blot, and library construction.
Kit composition:
Component
Can process 50ml blood
Can process 200ml blood
Buffer FG1
2×65ml
2×260ml
Buffer FG2
30ml
120ml
Buffer GE
15ml
60ml
Proteinase K
3 mg
12.5mg
Proteinase K storage solution
1.25ml
1.25ml
Storage conditions: Buffer FG1 2～8℃, other components at room temperature (15～30℃).
Features
1. Simple and fast: You can get high-quality DNA in about 15 minutes.
2. High purity: the extracted genomic DNA can be directly used in various experiments such as downstream PCR, fluorescent quantitative PCR, restriction enzyme digestion, etc.
3. Large extraction volume: DNA can be extracted from 0.1-20ml of whole blood,
4. Strong compatibility: suitable for processing various blood and cell samples.
5. Safe and non-toxic: do not use phenol, chloroform and other harmful substances.
Self-provided reagents: isopropanol, 70% ethanol
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Blood Genome Extraction Kit
